汪仁(博士,研究员)

      男,1975年生。博士,研究员,硕士研究生生导师,江苏省“333”高层次培养人才中青年学术技术带头人,全国农业生物化学与分子生物学会理事。现任江苏省中国科学院植物研究所天然产物化学研究中心副主任,植物次生代谢生物工程研究组负责人。

      主要研究方向为次生代谢产物的生物工程研究,包括:

  • 重要药用植物石蒜等的次生代谢物的合成生物学及其分子调控研究;
  • 药用植物内生菌的鉴定及其次生代谢物研究
  • 生物工程方法开发药用及其他重要化学原料的生产工艺研究。

      联系方式:

      E-mail:wangren@126.com 

                  rwang@cnbg.net

      电   话:025-84347111

成果简介


      近年来主持参与国家、省部级各类科研项目20余项,在《BMC Genomics》、 《Frontier in Plant Science》、 《Plant Molecular Biology》、 《Plos one》、 《Plant and Siol》、《Plant Physiology and Biochemistry》、 《Molecular Biology Reports》、 《Journal Integrative of Plant Biology》等刊物上发表论文80余篇(其中SCI论文40篇),申请、获授权国家发明专利22项,获江苏省科技进步奖二等奖1项,制定省级地方标准2项。

      近期主要承担的科研项目:

(1)国家自然科学基金面上项目,31770387,石蒜科生物碱合成关键C-C酚耦合酶细胞色素P450s的分离与功能分析,2018.1-2021.12

(2)国家自然科学基金面上项目,31572151,茉莉酸甲酯高效调控石蒜加兰他敏生物合成的分子机制,2016.1-2019.12。

(3)国家自然科学基金面上项目,31270339,基于转录组测序筛选石蒜加兰他敏生物合成关键酶基因及功能鉴定,2013.1-2016.12。

(4)国家高技术研究发展计划(863计划)子课题,2011AA10A206,植物生长调节剂、生物除草剂研究与产品创制,2011.1月-2015.12。

(5)江苏省第四期“333工程”培养资金项目,醇腈酶基因在红花石蒜抗旱中的作用及其分子机制研究,2015.7-2017.7。

(6)江苏省科技项目,BM2015019,药用植物石蒜天然产物加兰他敏合成及其调控机制研究,2015.6-2018.6。

(7)江苏省科技成果转化专项资金项目,BA2012074,国家基本药物胞磷胆碱钠制备工艺提升的研发及产业化,2012.9-2015.8。

(8)江苏省产学研联合创新资金--前瞻性联合研究项目,BY2014131,石蒜加兰他敏重要合成酶基因克隆及应用关键技术研究,2014.7 -2016.6

(9)江苏省科技支撑计划—社会发展,BE2012751,神经保护药胞磷胆碱基因工程产业化制备关键技术研究,2012.7-2015.6。


      代表性论文论著(*为通讯作者):

1) Wang Rong, Xu Sheng, Wang Ning, Xia Bing, Jiang Yumei, Wang Ren*. Transcriptome analysis of secondary metabolism pathway, transcription factors and transporters in response to methyl jasmonate in Lycoris aurea. Frontiers in Plant Science, 2017, 7: 1971
2) Xu Sheng, Sun Bin, Wang Rong, He Jia, Xia Bing, Xue Yong, Wang Ren*. Overexpression of a bacterial mercury transporter MerT in Arabidopsis enhances mercury tolerance. Biochemical and Biophysical Research Communications, 2017, 490(2):528-534
3) Xu Sheng, Jiang Yumei, Wang Ning, Xia Bing, Jiang Yilong, Li Xiaodan, Zhang Zhengzhi, Li Yikui, Wang Ren*. Identification and differential regulation of microRNAs in response to methyl jasmonate treatment in Lycoris aurea by deep sequencing. BMC Genomics, 2016, 17:789
4) Ma Rui, Xu Sheng, Zhao Yucheng, Xia Bing, Wang Ren*. Selection and validation of appropriate reference genes for quantitative Real-Time PCR analysis of gene expression in Lycoris aurea. Frontiers in Plant Science, 2016, 7: 536-551
5) Xu Sheng, Jiang Mingmin, Fu Jiangyan, Liang Lijian, Xia Bing, Wang Ren*. Physiological and Antioxidant Parameters in Two Lycoris Species as Influenced by Water Deficit Stress. HortScience, 2015, 50(11): 1702-1708
6) Ma F, Wang LJ, Li JL, Summa MK, Xie YJ, Wang R, Wang J, Zhang J, Shen WB. Interaction between HY1 and H2O2 in auxin‑induced lateral root formation in Arabidopsis. Plant Molecular Biology 2014, 85(1-2): 49-61
7) Jiang Yumei, Xia Bing, Liang Lijian, Li Xiaodan, Xu Sheng, Peng Feng, Wang Ren*. Molecular and analysis of a phenylalanine ammonia-lyase gene (LrPAL2) from Lycoris radiate. Molecular Biology Reports 2013, 40(3) :2293-2300
8) Li Xiaodan, Xia Bing, Wang Ren*, Xu Sheng, Jiang Yumei, Yu Fangbo*, Peng Feng. Molecular cloning and characterization of S-adenosylmethionine synthetase gene from Lycoris radiata. Molecular Biology Reports 2013, 40(2):1255-1263
9) Wang Ren*, Xu Sheng, Jiang Yumei, Jiang Jinwei, Li Xiaodan, Liang Lijian, He Jia, Peng Feng, Xia Bing. De novo sequence assembly and characterization of Lycoris aurea transcriptome using GS FLX titanium platform of 454 pyrosequencing. PLoS One, 2013, 8(4): e60449. 
10) Xu Sheng, Wang Lijuan, Zhang Bo, Han Bin, Xie Yanjie, Yang Jie, Zhong Weigong, Chen Huiping, Wang Ren, Shen Wenbiao*. RNAi knockdown of rice SE5 gene is sensitive to the herbicide methyl viologen by the down-regulation of antioxidant defense. Plant Molecular Biology, 2012, 80:219–235
11) Fu Guangqing, Xu Sheng, Xie Yanjie, Han Bin, Nie Li, Shen Wenbiao*, Wang Ren*. Molecular cloning, characterization, and expression of an alfalfa (Medicago sativa L.) heme oxygenase-1 gene, MsHO1, which is pro-oxidants-regulated. Plant Physiology and Biochemistry, 2011, 49: 792-799 
12) Jiang Yumei, Xia Nan, Li Xiaodan, Shen Wenbiao, Liang Lijian, Wang Chunyan, Wang Ren*, Peng Feng, Xia Bing*. Molecular cloning and characterization of a phenylalanine ammonia-lyase gene (LrPAL) from Lycoris radiate. Molecular Biology Reports 2011, 38:1935-1940
13) Li Xiaodan, Xia Bing, Jiang Yumei, Wu Qingsong, Wang Chunyan, He Lisi, Peng Feng, Wang Rrn*. A new pathogenesis-related protein, LrPR4, from Lycoris radiata, and its antifungal activity against Magnaporthe grisea. Molecular Biology Reports, 2010, 37: 995-1001
14) Mu Hongmei, Wang Ren*, Li Xiaodan, Jiang Yumei, Peng Feng, Xia Bing*. Alkaloid Accumulation in Different Parts and Ages of Lycoris chinensis. Zeitschrift für Naturforschung C 2010, 65c: 458-462
15) Wang Ren*, He Lisi, Xia Bing, Tong Jinfeng, Li Ning, Peng Feng. A micropropagation system for cloning of hemp (Cannabis sativa L.), by shoot tip culture. Pakistan Journal of Botany, 2009, 41(2): 603-608
16) Wang Ren, Shen Wenbiao, Liu Linglong, Jiang Ling, Liu Yuqiang, Su Ning, Wan Jianmin*. A novel lipoxygenase gene from developing rice seeds confers dual position specificity and responds to wounding and insect attack. Plant Molecular Biology, 2008, 66: 401-414

        其他成果:
1) 汪仁,李晓丹,夏冰,江玉梅,王忠. 胞苷三磷酸的基因工程制备方法. 专利号:ZL201110094643.3
2) 江玉梅,汪仁,夏冰,徐晟,贺佳,李晓丹,彭峰. 一种快速石蒜转基因的方法. 专利号:ZL20140244493.3
3) 夏冰, 汪仁, 李晓丹, 江玉梅, 彭峰, 王春燕, 何丽斯. 一种抗稻瘟病菌的石蒜属植物蛋白(LrPR4)及其编码基因与应用. 专利号:ZL200810124655.4
4) 汪仁,李宜奎,李晓丹,钱彬彬,徐晟,程丽,夏冰. 石蒜科植物忽地笑细胞色素P450还原酶2及其编码基因与应用. 专利申请号: 201611271566.3
5) 汪仁,李宜奎,钱彬彬,徐晟,李晓丹,程丽,夏冰. 一种对香豆酸-3-羟化酶及其编码基因与应用. 专利申请号: 201710098484.1
6) 汪仁,徐晟,傅江燕,李宜奎,夏冰. 一种山桃醇腈酶PdHNL2及其编码基因与应用. 专利申请号:201710232226.8
7) 汪仁,李晓丹,徐晟,夏冰,田开洋,谈金忠,徐成华,严刚,薛仁忠. 一种来源于枯草芽孢杆菌的质膜铝抗性基因在培育耐铝拟南芥中的应用. 申请号:201511027874.7
8) 汪仁,李晓丹,李宜奎,周佳宇,夏冰. 一株忽地笑内生细菌伯克霍尔德氏菌HDXY-02及其应用. 专利号:ZL 20171041783084